Induction of IL-8 and reactive oxygen species in periodontal ligament cells by Aggregatibacter actinomycetemcomitans / 대한치주과학회지
The Journal of the Korean Academy of Periodontology
; : 331-337, 2009.
Article
in Ko
| WPRIM
| ID: wpr-183844
Responsible library:
WPRO
ABSTRACT
PURPOSE: Interleukin (IL)-8 is one of pro-inflammatory cytokines. Reactive oxygen species (ROS) are reduced metabolites of O2. Aggregatibacter actinomycetemcomitans is one of representative periodontopathogens. To investigate the role of A. actinomycetemcomitans in IL-8 expression of periodontal ligament (PDL) cells, we estimated the production of IL-8 and ROS in A. actinomycetemcomitans treated PDL cells. METHODS: The IL-8 production was determined by enzyme-linked immunosorbent assay. The ROS production was estimated using H2DCFDA and FACS. RESULTS: A. actinomycetemcomitans increased the production of IL-8 and ROS at 10, 100, and 500 multiplicity of infection. N-cetylcysteine, an antioxidant of ROS, down-regulated the production of IL-8 induced by A. actinomycetemcomitans. CONCLUSION: These results suggest that A. actinomycetemcomitans induces IL-8 production and ROS may act as a mediator in this process.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Periodontal Ligament
/
Enzyme-Linked Immunosorbent Assay
/
Cytokines
/
Interleukin-8
/
Interleukins
/
Reactive Oxygen Species
/
Fluoresceins
Language:
Ko
Journal:
The Journal of the Korean Academy of Periodontology
Year:
2009
Type:
Article