Comparison of the Novel Human Papillomavirus 4 Auto-capillary Electrophoresis Test with the Hybrid Capture 2 Assay and with the PCR HPV Typing Set Test in the Detection of High-Risk HPV Including HPV 16 and 18 Genotypes in Cervical Specimens
Journal of Korean Medical Science
; : 579-584, 2009.
Article
in En
| WPRIM
| ID: wpr-185538
Responsible library:
WPRO
ABSTRACT
The aim of this study was to compare the novel human papillomavirus (HPV) detection method, the HPV 4 Auto-capillary Electrophoresis (ACE) test with the hybrid capture (HC) 2 assay for the detection of high-risk HPVs. In addition, we compared the HPV 4 ACE test with the polymerase chain reaction HPV Typing Set test for the detection of HPV 16 and HPV 18 genotypes. One hundred ninety-nine cervical swab samples obtained from women with previous abnormal Pap smears were subjected to testing with the three HPV tests. The HPV 4 ACE test and the HC 2 assay showed substantial agreement for detection of high-risk HPVs (85.4%, kappa=0.71). The HPV 4 ACE test also showed substantial agreement with the PCR HPV Typing Set test in the detection of HPV 16 and HP V 18 genotypes (89.9%, kappa=0.65). In correlation with cytologic results, the sensitivities and specificities of the HPV 4 ACE test and HC 2 assay were 92.9% vs. 92.9% and 48.1% vs. 50.8%, respectively, when high-grade squamous intraepithelial lesions were regarded as abnormal cytologies. The novel HPV 4 ACE test is a valuable tool for the detection of high-risk HPVs and for genotyping of HPV 16 and HPV 18.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Reagent Kits, Diagnostic
/
Vaginal Smears
/
DNA, Viral
/
Cervix Uteri
/
Polymerase Chain Reaction
/
Electrophoresis, Capillary
/
Human papillomavirus 16
/
Human papillomavirus 18
/
Gammapapillomavirus
/
Genotype
Type of study:
Diagnostic_studies
/
Etiology_studies
Limits:
Female
/
Humans
Language:
En
Journal:
Journal of Korean Medical Science
Year:
2009
Type:
Article