The time-course and RNA interference of TNF-alpha, IL-6, and IL-1beta expression on neuropathic pain induced by L5 spinal nerve transection in rats / 대한마취과학회지
Korean Journal of Anesthesiology
; : 159-169, 2015.
Article
in En
| WPRIM
| ID: wpr-190106
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
The objective of this study was to investigate the time-course of the expression of TNF-alpha, IL-6, and IL-1beta after L5 spinal nerve transection (SNT), and to determine the effect of small interfering RNA (siRNA) targeting these cytokines on neuropathic pain.METHODS:
Rats received control siRNA (CON group, n = 80) or a cocktail of siRNAs targeting these cytokines (COCK group, n = 70). The siRNAs were given via intrathecal catheter 1 d prior to SNT, on the operation day, and 1, 2 and 3 d postoperatively. Behavioral tests and levels of the cytokine mRNAs and proteins as well as glial cell activity were following the L5 SNT.RESULTS:
In the CON group, TNF-alpha and IL-1beta mRNA levels increased immediately after SNT and remained high for 6 d, while IL-6 transcripts only began to increase after 12 h. TNF-alpha and IL-1beta mRNA levels in the COCK group were lower than in the CON group at all time points (P < 0.05). In the behavioral tests, allodynia and hyperalgesia were significantly lower in the COCK group from 2 d after SNT (P < 0.05).CONCLUSIONS:
The time courses of TNF-alpha, IL-6 and IL-1beta mRNA expression after L5 SNT differ. RNA interference may be a method of reducing the development of mechanical allodynia and hyperalgesia in response to nerve injury.Key words
Full text:
1
Index:
WPRIM
Main subject:
Spinal Nerves
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RNA, Messenger
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Neuroglia
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Cytokines
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Interleukin-6
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Tumor Necrosis Factor-alpha
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RNA, Small Interfering
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RNA Interference
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Catheters
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Hyperalgesia
Limits:
Animals
Language:
En
Journal:
Korean Journal of Anesthesiology
Year:
2015
Type:
Article