Genotyping of Giardia duodenalis Isolates from Dogs in Guangdong, China Based on Multi-Locus Sequence
The Korean Journal of Parasitology
; : 299-304, 2014.
Article
in En
| WPRIM
| ID: wpr-190463
Responsible library:
WPRO
ABSTRACT
This study aimed to identify the assemblages (or subassemblages) of Giardia duodenalis by using normal or nested PCR based on 4 genetic loci glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), beta-giardin (bg), and small subunit ribosomal DNA (18S rRNA) genes. For this work, a total of 216 dogs' fecal samples were collected in Guangdong, China. The phylogenetic trees were constructed with MEGA5.2 by using the neighbor-joining method. Results showed that 9.7% (21/216) samples were found to be positive; moreover, 10 samples were single infection (7 isolates assemblage A, 2 isolates assemblage C, and 1 isolate assemblage D) and 11 samples were mixed infections where assemblage A was predominant, which was potentially zoonotic. These findings showed that most of the dogs in Guangdong were infected or mixed-infected with assemblage A, and multi-locus sequence typing could be the best selection for the genotype analysis of dog-derived Giardia isolates.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Phylogeny
/
Triose-Phosphate Isomerase
/
Molecular Sequence Data
/
RNA, Ribosomal, 18S
/
Cluster Analysis
/
China
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Polymerase Chain Reaction
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DNA, Protozoan
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Giardiasis
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Giardia lamblia
Limits:
Animals
Country/Region as subject:
Asia
Language:
En
Journal:
The Korean Journal of Parasitology
Year:
2014
Type:
Article