Cloning and Iron Transportation of Nucleotide Binding Domain of Cryptosporidium andersoni ATP-Binding Cassette (CaABC) Gene
The Korean Journal of Parasitology
; : 335-339, 2015.
Article
in En
| WPRIM
| ID: wpr-19167
Responsible library:
WPRO
ABSTRACT
Cryptosporidium andersoni ATP-binding cassette (CaABC) is an important membrane protein involved in substrate transport across the membrane. In this research, the nucleotide binding domain (NBD) of CaABC gene was amplified by PCR, and the eukaryotic expression vector of pEGFP-C1-CaNBD was reconstructed. Then, the recombinant plasmid of pEGFP-C1-CaNBD was transformed into the mouse intestinal epithelial cells (IECs) to study the iron transportation function of CaABC. The results indicated that NBD region of CaABC gene can significantly elevate the transport efficiency of Ca2+, Mg2+, K+, and HCO3 - in IECs (P<0.05). The significance of this study is to find the ATPase inhibitors for NBD region of CaABC gene and to inhibit ATP binding and nutrient transport of CaABC transporter. Thus, C. andersoni will be killed by inhibition of nutrient uptake. This will open up a new way for treatment of cryptosporidiosis.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Molecular Sequence Data
/
Protozoan Proteins
/
Adenosine Triphosphate
/
Calcium
/
Sequence Alignment
/
Amino Acid Sequence
/
Cloning, Molecular
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Protein Structure, Tertiary
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ATP-Binding Cassette Transporters
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Cryptosporidiosis
Limits:
Animals
/
Humans
Language:
En
Journal:
The Korean Journal of Parasitology
Year:
2015
Type:
Article