The Effect of Vasoactive Intestinal Peptide on Cord Blood CD34 (+) Cells / 대한소아혈액종양학회지
Korean Journal of Pediatric Hematology-Oncology
; : 262-268, 2003.
Article
in Ko
| WPRIM
| ID: wpr-194907
Responsible library:
WPRO
ABSTRACT
PURPOSE:
We investigated the expression of vasoactive intestinal peptide (VIP), VIP receptor 1 (VPAC1), VIP receptor 2 (VPAC2) genes in the human umbilical cord blood CD34 cells, and the ability of VIP to stimulate human primitive as well as monopotent hematopoietic progenitors.METHODS:
We isolated RNA from umbilical cord blood CD34 cells, and then performed RT-PCR, and sequencing. The umbilical cord blood CD34 cells were cultured with the various concentrations of VIP for burst-forming unit of erythrocyte (BFU-E), colony-forming unit of granulocyte/monocyte (CFU-GM), colony-forming unit of graulocyte/erythrocyte/monocyte/megakaryocyte (CFU-GEMM), and colony-forming unit of megakaryocyte (CFU-Mk).RESULTS:
The RNA coding for VPAC1 was detected in human umbilical cord blood CD34 cells. VIP significantly stimulated the growth of CFU-GEMM and CFU-Mk.CONCLUSION:
The present results suggest that VIP is an important neuropeptide in the early proliferation of human primitive as well as megakaryocyte progenitors.Key words
Full text:
1
Index:
WPRIM
Main subject:
Stem Cells
/
Neuropeptides
/
RNA
/
Megakaryocytes
/
Vasoactive Intestinal Peptide
/
Receptors, Vasoactive Intestinal Peptide
/
Myeloid Progenitor Cells
/
Erythrocytes
/
Megakaryocyte Progenitor Cells
/
Fetal Blood
Limits:
Humans
Language:
Ko
Journal:
Korean Journal of Pediatric Hematology-Oncology
Year:
2003
Type:
Article