Matrix metalloproteinase-8 and substance P levels in root canal exudates of nonvital teeth / 대한치과보존학회지
Journal of Korean Academy of Conservative Dentistry
; : 196-202, 2011.
Article
in Ko
| WPRIM
| ID: wpr-19516
Responsible library:
WPRO
ABSTRACT
OBJECTIVES:
The aim of this study was to investigate levels of matrix metalloproteinase-8 (MMP-8) and substance P (SP) in root canal exudates during root canal treatment (RCT) of nonvital, painful teeth. MATERIALS ANDMETHODS:
Patients scheduled for nonsurgical RCT were prospectively selected; the study was performed after obtaining informed consent from the patients and was approved by the Institutional Review Board for Clinical Research of Gangnam Severance Hospital, Yonsei University (3-2008-0118). Canal exudates samples were collected using sterilized paper points from teeth scheduled for RCT across three different time periods. MMP-8 and SP levels were measured using enzyme-linked immunosorbent assay (ELISA). Data were analyzed using a mixed model analysis and the Pearson correlation analysis (p < 0.05).RESULTS:
MMP-8 and SP levels in GCF were decreased during RCT (p < 0.0001), and they showed a weak positive correlation to each other (p < 0.05). Patients'subjective pain levels and the response from percussion test were significantly related to SP level.CONCLUSIONS:
This study demonstrated that periradicular inflammation endodontic origin can elevate SP and MMP-8 levels in root canal exudates. Interestingly, SP level of canal exudates showed a possibility of being used as an indicator of pain due to periapical pathosis.Key words
Full text:
1
Index:
WPRIM
Main subject:
Percussion
/
Tooth
/
Enzyme-Linked Immunosorbent Assay
/
Substance P
/
Prospective Studies
/
Tooth, Nonvital
/
Matrix Metalloproteinase 8
/
Ethics Committees, Research
/
Dental Pulp Cavity
/
Exudates and Transudates
Type of study:
Observational_studies
Limits:
Humans
Language:
Ko
Journal:
Journal of Korean Academy of Conservative Dentistry
Year:
2011
Type:
Article