Correlation between the overexpression of epidermal growth factor receptor and mesenchymal makers in endometrial carcinoma / 부인종양
Journal of Gynecologic Oncology
; : 36-42, 2014.
Article
in En
| WPRIM
| ID: wpr-202950
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
The objective of this study was to evaluate the effect of overexpression of epidermal growth factor receptor (EGFR) on the expression of epithelial cell markers (E-cadherin and alpha-catenin) and mesenchymal cell markers (N-cadherin and vimentin) in endometrial carcinoma.METHODS:
The expression of all 4 markers was evaluated in EGFR overexpressing Ishikawa cells, control Ishikawa cells, and KLE cells using reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. The expression of these 4 markers was also determined in cancerous tissues of patients with endometrial carcinoma using immunohistochemical staining.RESULTS:
Ishikawa cells transfected with EGFR showed decreased expression of E-cadherin and alpha-catenin and increased expression of N-cadherin and vimentin compared with control Ishikawa cells (p<0.01 for all). The expression of N-cadherin and vimentin was higher and the expression of E-cadherin and alpha-catenin was lower in stage II-III than stage I and in grade II-III than grade I endometrial carcinoma tissue (p<0.01 for all).CONCLUSION:
Decreased expression of epithelial markers (E-cadherin and alpha-catenin) and increased expression of mesenchymal markers (N-cadherin and vimentin) were observed in human endometrial carcinoma tissue. These findings correlate with high EGFR expression in cultured endometrial carcinoma cells.Key words
Full text:
1
Index:
WPRIM
Main subject:
Vimentin
/
Cadherins
/
Blotting, Western
/
Polymerase Chain Reaction
/
Endometrial Neoplasms
/
Reverse Transcription
/
Epidermal Growth Factor
/
Epithelial Cells
/
Alpha Catenin
/
Epithelial-Mesenchymal Transition
Limits:
Female
/
Humans
Language:
En
Journal:
Journal of Gynecologic Oncology
Year:
2014
Type:
Article