Effect of a Flavon Extracted from Artemisia absinthium on Collagen Induced Arthritis in Mice / 소아과
Korean Journal of Pediatrics
; : 677-684, 2004.
Article
in En
| WPRIM
| ID: wpr-203173
Responsible library:
WPRO
ABSTRACT
PURPOSE: In this study, a possible suppressive effect of a flavon extracted from Artemisia absinthium on a mouse collagen-induced arthritis (CIA) model was investigated. METHODS: DBA/1 mice were injected intradermally with emulsified chicken type II collagen. Three weeks after immunization, a flavon was introduced p.o. everyday. Clinical incidences of arthritis and arthritis index were measured. Measurement of anti-collagen antibodies and a stimulation index of the splenocytes of the mice were measured. IL-10 and TNF-alpha in the supernatants of the mice sera were measured by ELISA. mRNA expression for IL-10 and TNF-alpha in the splenocytes were tested. RESULTS: Flavon extracted from Artemisia absinthium appears to be an effective suppressor of CIA in mice. The serum anti-collagen antibody level and stimulation index of the cultured splenocytes showed no significant differences among the three experimental groups. Also serum IL-10 and TNF-alpha levels did not show any significant differences among the three experimental groups. An increased expression of mRNA for IL-10 was observed in the splenocytes treated with flavon. CONCLUSION: With these results, flavon extracted from Artemisia absinthium appears to have a suppressive effect of CIA. The mechanism of the suppressive effect of flavon extracted from Artemisia absinthium may be from a stimulation of IL-10 production.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Arthritis
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Arthritis, Experimental
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RNA, Messenger
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Enzyme-Linked Immunosorbent Assay
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Chickens
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Incidence
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Collagen
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Immunization
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Tumor Necrosis Factor-alpha
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Interleukin-10
Type of study:
Incidence_studies
/
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Korean Journal of Pediatrics
Year:
2004
Type:
Article