Atractylochromene Is a Repressor of Wnt/beta-Catenin Signaling in Colon Cancer Cells
Biomolecules & Therapeutics
; : 26-30, 2015.
Article
in En
| WPRIM
| ID: wpr-20364
Responsible library:
WPRO
ABSTRACT
Wnt/beta-catenin signaling pathway was mutated in about 90% of the sporadic and hereditary colorectal cancers. The abnormally activated beta-catenin increases the cancer cell proliferation, differentiation and metastasis through increasing the expression of its oncogenic target genes. In this study, we identified an inhibitor of beta-catenin dependent Wnt pathway from rhizomes of Atractylodes macrocephala Koidzumi (Compositae). The active compound was purified by activity-guided purification and the structure was identified as 2,8-dimethyl-6-hydroxy-2-(4-methyl-3-pentenyl)-2H-chromene (atractylochromene, AC). AC suppressed beta-catenin/T-cell factor transcriptional activity of HEK-293 reporter cells when they were stimulated by Wnt3a or inhibitor of glycogen synthase kinase-3beta. AC down-regulated the nuclear level of beta-catenin through the suppression of galectin-3 mediated nuclear translocation of beta-catenin in SW-480 colon cancer cells. Furthermore, AC inhibits proliferation of colon cancer cell. Taken together, AC from A. macrocephala might be a potential chemotherapeutic agent for the prevention and treatment of human colon cancer.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Colorectal Neoplasms
/
Glycogen Synthase
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Colonic Neoplasms
/
Rhizome
/
Atractylodes
/
Galectin 3
/
Cell Proliferation
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Beta Catenin
/
Wnt Signaling Pathway
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Neoplasm Metastasis
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Biomolecules & Therapeutics
Year:
2015
Type:
Article