Expression of TIM-3, Human beta-defensin-2, and FOXP3 and Correlation with Disease Activity in Pediatric Crohn's Disease with Infliximab Therapy
Gut and Liver
;
: 370-380, 2015.
Article
in English
| WPRIM
| ID: wpr-203889
ABSTRACT
BACKGROUND/AIMS:
This study investigated the expression of T cell immunoglobulin- and mucin-domain-containing molecule 3 (TIM-3), human beta-defensin (HBD)-2, forkhead box protein 3 (FOXP3), and the frequency of CD4+ CD25+ FOXP3+ regulatory T cells (Tregs) in children with Crohn's disease (CD) during infliximab therapy.METHODS:
We enrolled 20 CD patients who received infliximab treatment for 1 year. Peripheral blood and colonic mucosal specimens were collected from all CD patients and from healthy control individuals.RESULTS:
A significant difference in TIM-3 mRNA expression was evident in peripheral blood mononuclear cells and colonic mucosa between CD patients before infliximab therapy and the healthy controls (p<0.001 and p=0.005, respectively). A significant difference in HBD-2 mRNA expression was found in colonic mucosa between CD patients before infliximab therapy and the healthy controls (p=0.013). In the active phase of CD, at baseline, the median percentage of T cells that were CD25+ FOXP3+ was 1.5% (range, 0.32% to 3.49%), which increased after inflixmab treatment for 1 year to 2.2% (range, 0.54% to 5.02%) (p=0.008).CONCLUSIONS:
Our study suggests that both the adaptive and innate immune systems are closely linked to each other in CD pathogenesis. And the results of our study indicate that it could be a useful therapeutic tool, where restoration of TIM-3, HBD-2 and the function of Tregs may repair the dysfunctional immunoregulation in CD.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Gastrointestinal Agents
/
Leukocytes, Mononuclear
/
Crohn Disease
/
Case-Control Studies
/
T-Lymphocytes, Regulatory
/
Colon
/
Beta-Defensins
/
Forkhead Transcription Factors
/
Infliximab
/
Intestinal Mucosa
Type of study:
Observational study
/
Risk factors
Limits:
Adolescent
/
Female
/
Humans
/
Male
Language:
English
Journal:
Gut and Liver
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS