Five Rare Non-Tuberculous Mycobacteria Species Isolated from Clinical Specimens / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases
; : 331-336, 2010.
Article
in Ko
| WPRIM
| ID: wpr-204139
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
Recently, the rate of infections with non-tuberculous mycobacteria (NTM) has been increasing in Korea. Precise identification of NTM is critical to determination of the pathogen and to target treatment of NTM patients.METHODS:
Sixty-eight unclassified mycobacteria isolates by rpoB PCR-RFLP assay (PRA) collected in 2008 were analyzed by National Center for Biotechnology Information (NCBI) Basic Local Alignment Search Tool (BLAST) search after sequencing of 16S rRNA, hsp65, rpoB genes.RESULTS:
Nineteen strains of 68 isolates were specified as species after sequencing analysis of 3 gene types. We found 3 M. lentifulavum, 5 M. arupense, 4 M. triviale, 4 M. parascrofulaceum, and one M. obuense. One M. tuberculosis and another M. peregrinum were mutated at the Msp I recognition site needed for rpoB PRA. The remaining 49 isolates did not coincide with identical species at the 3 kinds genes.CONCLUSION:
Sequencing analysis of 16S rRNA, hsp65, rpoB was useful for identification of NTM unclassified by rpoB PRA.Key words
Full text:
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Index:
WPRIM
Main subject:
Tuberculosis
/
Biotechnology
/
RNA, Ribosomal, 16S
/
Korea
/
Nontuberculous Mycobacteria
Country/Region as subject:
Asia
Language:
Ko
Journal:
Tuberculosis and Respiratory Diseases
Year:
2010
Type:
Article