Simple Purification of Adeno-Associated Virus-DJ for Liver-Specific Gene Expression
Yonsei Medical Journal
; : 790-794, 2016.
Article
in En
| WPRIM
| ID: wpr-205734
Responsible library:
WPRO
ABSTRACT
Recombinant gene expression using adeno-associated viruses (AAVs) has become a valuable tool in animal studies, as they mediate safe expression of transduced genes for several months. The liver is a major organ of metabolism, and liver-specific expression of a gene can be an invaluable tool for metabolic studies. AAV-DJ is a recombinant AAV generated by the gene shuffling of various AAV serotypes and shares characteristics of AAV2 and AAV8. AAV-DJ contains a heparin-binding domain in its capsid, which suggests that a heparin column could be used for the purification of the AAV. Given that AAV-DJ has been only recently available, relatively little is known about the optimal preparation/purification and application of AAV-DJ. Here, we present a simple large-scale preparation method that can generate 3×10(13) viral particles for in vivo experiments and demonstrate liver-specific gene expression via systemic injection in mice.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Gene Expression
/
Capsid
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Genome, Viral
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Dependovirus
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Capsid Proteins
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Hep G2 Cells
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Genetic Vectors
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Liver
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Mice, Inbred C57BL
Limits:
Animals
/
Humans
Language:
En
Journal:
Yonsei Medical Journal
Year:
2016
Type:
Article