Utility of In-House PCR for HLA-B27 Typing: Comparison of Concordance Rate between PCR Kit and In-House PCR / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
; : 239-243, 2008.
Article
in Ko
| WPRIM
| ID: wpr-206225
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
Commercial kits of PCR method are widely used in HLA-B27 typing; however, their cost is relatively high. In this study, we evaluated the utility of an in-house PCR method by comparing it with that of a commercial kit.METHODS:
HLA-B27 typing was done in 188 patients by using two PCR methods, Absolute(TM) HLAB27 PCR kit (Biosewoom, Korea) and an in-house PCR method. The primers used in the in-house method were prepared by Bioneer (Korea). Both PCR tests were done by Gene Amp PCR System 9600 (Perkin-Elmer Centus Corp., USA).RESULTS:
The commercial kit and in-house PCR showed 100% concordance rate with each other in HLA-B27 typing. Of 188 patients tested 72 (38.3%) were positive and 116 (61.7%) were negative by the both tests. Of 62 patients with ankylosing spondylitis, 50 were positive (80.7%).CONCLUSIONS:
The in-house PCR is a reliable and cost-effective method and can replace or supplement commercial kits for HLA-B27 typing.Key words
Full text:
1
Index:
WPRIM
Main subject:
Reagent Kits, Diagnostic
/
Histocompatibility Testing
/
HLA-B27 Antigen
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
Type of study:
Diagnostic_studies
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
Ko
Journal:
The Korean Journal of Laboratory Medicine
Year:
2008
Type:
Article