T Lymphocyte Subsets and Cytokines in Rats Transplanted with Adipose-Derived Mesenchymal Stem Cells and Acellular Nerve for Repairing the Nerve Defects
Journal of Korean Neurosurgical Society
;
: 101-106, 2015.
Article
in English
| WPRIM
| ID: wpr-211053
ABSTRACT
OBJECTIVE:
The aim of this study was to explore the immunity in rats transplanted with adipose-derived mesenchymal stem cells (ADSCs) and acellular nerve (ACN) for repairing sciatic nerve defects.METHODS:
ADSCs were isolated from the adipose tissues of Wistar rats. Sprague-Dawley rats were used to establish a sciatic nerve defect model and then divided into four groups, according to the followingmethods:
Group A, allogenic nerve graft; Group B, allograft with ACN; Group C, allograft ADSCs+ACN, and Group D, nerve autograft.RESULTS:
At the day before transplantation and 3, 7, 14, and 28 days after transplantation, orbital venous blood of the Sprague-Dawley rats in each group was collected to detect the proportion of CD3+, CD4+, and CD8+ subsets using flow cytometry and to determine the serum concentration of interleukin-2 (IL-2), tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) using enzyme-linked immunosorbent assay (ELISA). At each postoperative time point, the proportion of CD3+, CD4+, and CD8+ subsets and the serum concentration of IL-2, TNF-alpha, and IFN-gamma in group C were all near to those in group B and group D, in which no statistically significant difference was observed. As compared with group A, the proportion of CD3+, CD4+, and CD8+ subsets and the serum concentration of IL-2, TNF-alpha, and IFN-gamma were significantly reduced in group C (p<0.05).CONCLUSION:
The artificial nerve established with ADSCs and ACN has no obvious allograft rejection for repairing rat nerve defects.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Orbit
/
Sciatic Nerve
/
Enzyme-Linked Immunosorbent Assay
/
Cytokines
/
T-Lymphocyte Subsets
/
Interferon-gamma
/
Interleukin-2
/
Tumor Necrosis Factor-alpha
/
Rats, Wistar
/
Rats, Sprague-Dawley
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Journal of Korean Neurosurgical Society
Year:
2015
Type:
Article
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