Comparison of 3 Automated Immunoassays for Detection of Anti-Hepatitis A Virus Immunoglobulin M in a Tertiary Care Hospital
Annals of Laboratory Medicine
; : 121-124, 2013.
Article
in En
| WPRIM
| ID: wpr-216012
Responsible library:
WPRO
ABSTRACT
Three automated immunoassay kits for anti-Hepatitis A Virus (HAV) IgM-Architect, (Abbott Laboratories, USA), Elecsys (Roche Diagnostics, Germany), and ADVIA Centaur (Siemens Healthcare Diagnostics Inc., USA)-were compared. We included 178 consecutive samples, for which an anti-HAV IgM test was requested at Seoul National University Hospital from September 2009 to January 2010. Reviewing of medical records, reverse transcription (RT)-PCR for HAV RNA, or total anti-HAV assay were performed on 16 (9.0%) samples with discrepant results. The percent agreements (kappas) of the Architect and ADVIA Centaur, Architect and Elecsys, and ADVIA Centaur and Elecsys kits were 96.6% (0.91), 96.6% (0.92), and 97.8% (0.94), respectively. Eight out of 16 discrepant samples showed gray-zone values in Architect but were nonreactive in the others. Slightly earlier seroconversion was suspected in Elecsys. The 3 assays showed comparable performances with excellent agreements in a tertiary care hospital setting.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Reagent Kits, Diagnostic
/
Tertiary Healthcare
/
Immunoglobulin M
/
RNA, Viral
/
Immunoassay
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Retrospective Studies
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Reverse Transcriptase Polymerase Chain Reaction
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Hepatitis A virus
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Hepatitis B
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Hospitals, University
Type of study:
Diagnostic_studies
/
Observational_studies
Limits:
Humans
Language:
En
Journal:
Annals of Laboratory Medicine
Year:
2013
Type:
Article