A Case of the False-negative D Phenotype in a Neonate with a Strongly Positive Direct Antiglobin Test Rest
Laboratory Medicine Online
; : 45-49, 2016.
Article
in Ko
| WPRIM
| ID: wpr-220315
Responsible library:
WPRO
ABSTRACT
Accurate D antigen blood typing is needed owing to the clinical importance of the Rh blood group. We describe a female infant who was suspected to suffer from Rh incompatible hemolytic disease of the newborn, and who showed a strong positive direct antiglobin test (DAT) result and false red blood cell (RBC) agglutination in D typing. Using chloroquine dissociation of IgG, we confirmed that the antibodies coating her RBCs were of anti-D type. D typing with 0.8% RBC suspensions in saline using saline gel cards showed 2+ RBC agglutinations. After increasing the incubation time of dissociation by chloroquine for up to 4 hr, the dissociated RBCs began to show agglutination in both the tube technique (2+) and the gel card technique (4+) for D typing, although the DAT rest was still positive. Therefore, in order to prevent mistyping as a false-negative D blood group, whenever the D blood typing of a patient with a strong positive DAT rest does not show RBC agglutination, retesting of the D blood typing is recommended by using saline-suspended RBCs or dissociated RBCs.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Phenotype
/
Suspensions
/
Immunoglobulin G
/
Blood Grouping and Crossmatching
/
Chloroquine
/
Agglutination
/
Erythrocytes
/
Antibodies
Limits:
Female
/
Humans
/
Infant
/
Newborn
Language:
Ko
Journal:
Laboratory Medicine Online
Year:
2016
Type:
Article