Curcumin treatment recovery the decrease of protein phosphatase 2A subunit B induced by focal cerebral ischemia in Sprague-Dawley rats / 한국실험동물학회지
Laboratory Animal Research
;
: 134-138, 2015.
Article
in English
| WPRIM
| ID: wpr-223859
ABSTRACT
Curcumin provides various biological effects through its anti-inflammatory and antioxidant properties. Moreover, curcumin exerts a neuroprotective effect against ischemic condition-induced brain damage. Protein phosphatase 2A (PP2A) is a ubiquitous serine and threonine phosphatase with various cell functions and broad substrate specificity. Especially PP2A subunit B plays an important role in nervous system. This study investigated whether curcumin regulates PP2A subunit B expression in focal cerebral ischemia. Cerebral ischemia was induced surgically by middle cerebral artery occlusion (MCAO). Adult male rats were injected with either vehicle or curcumin (50 mg/kg) 1 h after MCAO and cerebral cortex tissues were isolated 24 h after MCAO. A proteomics study, reverse transverse-PCR and Western blot analyses were performed to examine PP2A subunit B expression levels. We identified a reduction in PP2A subunit B expression in MCAO-operated animals using a proteomic approach. However, curcumin treatment prevented injury-induced reductions in PP2A subunit B levels. Reverse transverse-PCR and Western blot analyses confirmed that curcumin treatment attenuated the injury-induced reduction in PP2A subunit B levels. These findings can suggest that the possibility that curcumin maintains levels of PP2A subunit B in response to cerebral ischemia, which likely contributes to the neuroprotective function of curcumin in cerebral ischemic injury.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Serine
/
Substrate Specificity
/
Brain
/
Cerebral Cortex
/
Brain Ischemia
/
Blotting, Western
/
Rats, Sprague-Dawley
/
Phosphoprotein Phosphatases
/
Neuroprotective Agents
/
Infarction, Middle Cerebral Artery
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Laboratory Animal Research
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS