Metallothionein 1 isoform gene expression induced by cadmium in human peripheral blood lymphocytes / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 104-109, 2006.
Article
in En
| WPRIM
| ID: wpr-229719
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the gene expression of metallothionein 1 (MT-1) isoforms in human peripheral blood lymphocytes (HPBLs).</p><p><b>METHODS</b>The expression of mRNA representing the seven active MT-1 genes was determined in HPBLs by quantitative RT-PCR before and after exposure to cadmium.</p><p><b>RESULTS</b>Basal expressions of MT-1X, and MT-1A in HPBLs were similar to expression of housekeeping gene. In contrast, the basal gene expressions of MT-1H, 1F, 1E, and 1G were a little transcripts in human HPBLs. No signal was detected for MT-1B. There was a sex difference (P < 0.05). in basal gene expression of MT-1E. The levels of gene expression of MT-1A, 1E, 1F, 1G, 1H, and 1X increased, but the level of MT-1B did not increase after exposure to cadmium.</p><p><b>CONCLUSIONS</b>Gene expressions of MT-1G, MT-1H, MT-1F, and MT-1X in HPBLs can be used as a potential biomarker of cadmium exposure.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Cadmium
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Lymphocytes
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Biomarkers
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Cells, Cultured
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Gene Expression Regulation
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DNA Primers
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Protein Isoforms
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Reverse Transcriptase Polymerase Chain Reaction
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Genetics
Limits:
Adult
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Female
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Humans
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Male
Language:
En
Journal:
Biomedical and Environmental Sciences
Year:
2006
Type:
Article