The methylation locus and frequency pattern on p16 INK4a gene promoter CpG in epidermis of patients with psoriasis / 中华医学遗传学杂志
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
; (6): 674-676, 2007.
Article
in Zh
| WPRIM
| ID: wpr-229847
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the CpG methylation locus and frequency pattern on p16 INK4a gene promoter in epidermis of p16 INK4a methylated patients with psoriasis vulgaris.</p><p><b>METHODS</b>The DNA specimens were obtained from epidermal lesion of 50 plaque psoriatic patients. Methylation specific PCR and DNA sequencing were used to detect the frequency and locus of methylation in p16 INK4a gene promoter region.</p><p><b>RESULTS</b>Approximately 50% CpG was methylated in p16 INK4a methylated patients, methylation was found in specifical locus of p16 INK4a gene promoter.</p><p><b>CONCLUSION</b>The distinct methylation pattern is showed on the p16 INK4a gene promoter region in patients with psoriasis.</p>
Full text:
1
Index:
WPRIM
Main subject:
Psoriasis
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Molecular Sequence Data
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Base Sequence
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Polymerase Chain Reaction
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Sequence Alignment
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Promoter Regions, Genetic
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CpG Islands
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DNA Methylation
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Cyclin-Dependent Kinase Inhibitor p16
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Tumor Suppressor Protein p14ARF
Limits:
Adolescent
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Adult
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Aged
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Female
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Humans
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Male
Language:
Zh
Journal:
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
Year:
2007
Type:
Article