Mitogen-activated protein kinase genes of Artemisia annua and their expression analysis under Cadmium stress / 中国中药杂志
Zhongguo Zhong Yao Za Zhi
; (24): 1016-1020, 2016.
Article
in Zh
| WPRIM
| ID: wpr-230043
Responsible library:
WPRO
ABSTRACT
In order to study Artemisia annua under cadmium stress, whether there are corresponding MAPK genes involved in transduction of the cadmium signal. 17 AaMAPK genes, named AaMAPK1-AaMAPK17 repectively, were finally obtained by using Trinity method for de novo assembly of transcripts from SRA database and BLAST search against AtMAPK genes and determing conserved domain using a series of bioinformatics tools. There exist 16 MAPK genes contained T[D/E]Y conserved domains among the obtained genes. The expressions of these genes were analyzed by Real-time PCR under cadmium stress. The results showed that the expressions level of AaMAPK3 and AaMAPK10 were down-regulated and MAPK7, MAPK9 and MAPK12 were up-regulated. These indicated that there exist corresponding MAPK genes involved in transduction of the cadmium signal.
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WPRIM
Language:
Zh
Journal:
Zhongguo Zhong Yao Za Zhi
Year:
2016
Type:
Article