Influence of Interferon alpha-2b on proliferation inhibition and apoptosis induction in HL-60 cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 56-58, 2007.
Article
in Zh
| WPRIM
| ID: wpr-230334
Responsible library:
WPRO
ABSTRACT
To investigate the effects of interferon alpha-2b on proliferation and apoptosis in HL-60 cells, HL-60 cells were cultured in different concentrations of IFN alpha-2b. The morphologic changes were observed by Wright's and acridine orange (AO) and ethidium bromide (EB) staining respectively. Inhibition of proliferation was detected by MTT. Expression of CD13(+) was checked by indirect fluoroimmunoassay. The results showed that apoptosis rate of HL-60 cells assayed by the above-mentioned two methods was (51 +/- 2)% and (78 +/- 3)% respectively and OD(570) values of proliferation inhibited were 1.8 +/- 0.1 and 1.0 +/- 0.1 respectively when the concentrations of the IFN(alpha-2b) were 500 and 10,000 U/ml in culture for 48 hours. Morphology and count of CD13(+) cells were changed. CD13(+) cell expression rate was (62 +/- 2)% and (30 +/- 3)% respectively when the concentrations of the IFN(alpha-2b) were 500 and 10,000 U/ml in culture for 48 hours. It is concluded that IFN(alpha-2b) can enhance the apoptosis of HL-60 cells, inhibit their proliferation, promote their maturation and differentiation.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Recombinant Proteins
/
Interferon-alpha
/
Apoptosis
/
CD13 Antigens
/
HL-60 Cells
/
Cell Proliferation
/
Genetics
/
Antineoplastic Agents
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2007
Type:
Article