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Study on production of acrylamide by microbial method (I)--Culture of bacterium cells and expression of high activity of nitrile hydratase / 生物工程学报
Article in Zh | WPRIM | ID: wpr-231373
Responsible library: WPRO
ABSTRACT
The cultural conditions for the growth of Norcardia cell were studied in this paper. Controlling pH value, adding nutrient and optimizing the quantity of inducer during cultivation, the activity of nitrile hydratase reached 6567 u/mL (culture medium), which was the highest value appeared in native journals. In the farther hydratase experiments, no by-product, crylic acid, was detected. It showed that the activity of amidase was not promoted obviously while the activity of nitrile hydratase was increased greatly. The results set a strong foundation for the industrial application and the research on new technology.
Subject(s)
Full text: 1 Index: WPRIM Main subject: Physiology / Acrylamides / Biotechnology / Cell Culture Techniques / Fermentation / Amidohydrolases / Glucose / Hydro-Lyases / Hydrogen-Ion Concentration / Metabolism Language: Zh Journal: Chinese Journal of Biotechnology Year: 2002 Type: Article
Full text: 1 Index: WPRIM Main subject: Physiology / Acrylamides / Biotechnology / Cell Culture Techniques / Fermentation / Amidohydrolases / Glucose / Hydro-Lyases / Hydrogen-Ion Concentration / Metabolism Language: Zh Journal: Chinese Journal of Biotechnology Year: 2002 Type: Article