Screening, identification and analysis of platelet differential functional proteins in patients with coronary heart disease of blood-stasis pattern / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 467-473, 2010.
Article
in Zh
| WPRIM
| ID: wpr-231499
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To seek the key platelet functional proteins (PFPs) for the occurrence of blood-stasis pattern (BP) in patients with coronary heart disease (CHD).</p><p><b>METHODS</b>Peripheral blood platelet protein of 22 patients and 24 healthy person (for control) were extracted respectively in 4 batches for carrying 4 times of the test out. Differential PFPs in samples were screened out by two-dimensional fluorescence difference gel electrophoresis, and identified with matrix-assisted laser desorption/ionization-time of flight mass spectrometry; then the identified proteins were further authenticated by Western-blotting.</p><p><b>RESULTS</b>Thirteen differential PFPs were screened out, and among them the 7 identified by spectrometry were: isoform 1 of integrin alpha- II b, isoform 2 of integrin alpha- II b, actin-cytoplasmic 1, actin-cytoplasmic 2, cDNA FLJ52842, cDNA FLJ55253, and cDNA FLJ43573 fis. Among them isoform 2 of integrin alpha- II b (CD41) and actin-cytoplasmic 2 (Acting) were authenticated successfully.</p><p><b>CONCLUSION</b>CD41 and acting are the possible marker proteins, and the other PFPs might play crucial roles in the occurrence and development of BSS in CHD.</p>
Full text:
1
Index:
WPRIM
Main subject:
Blood
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Blood Platelets
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Platelet Membrane Glycoproteins
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Electrophoresis, Gel, Two-Dimensional
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Case-Control Studies
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Coronary Disease
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Diagnosis
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Medicine, Chinese Traditional
Type of study:
Diagnostic_studies
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Observational_studies
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Screening_studies
Limits:
Adult
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Aged
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Female
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Humans
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Male
Language:
Zh
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2010
Type:
Article