Establishment of immortalized cell line BLTR-4 and primary identification of its biological character / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 543-548, 2004.
Article
in Zh
| WPRIM
| ID: wpr-231890
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish immortalized cell line from the urothelium of the urinary bladder and identify the characteristics of the cell line.</p><p><b>METHODS</b>Human papillomavirus 16 (HPV-16) plasmid was used to transfect urothelium of infant urinary bladder in vitro with the help of Fugene-6, and this plasmid contained E6 and E7 genes of HPV-16. We also identified the existence of HPV-16 E6 and E7 genes and the biological characteristics of the cell line by PCR, immunohistochemistry, and the biology identification.</p><p><b>RESULTS</b>BLTR-4 cell line, produced from the transfection of HPV-16K plasmid, was a cell line from urothelium with the expression of HPV-16 E6 and E7 genes. It had been cultured more than 70 passages, and the characteristics of growth was similar to the immortalized cell line as reported.</p><p><b>CONCLUSIONS</b>BLTR-4 cell line is an immortalized cell line from urothelium of the urinary bladder, which contains HPV-16 E6 and E7 genes. BLTR-4 cell line is a good experimental model to investigate the relationship of the infection of high risk HPV and transitional cell carcinoma (TCC) in vitro.</p>
Full text:
1
Index:
WPRIM
Main subject:
Papillomaviridae
/
Plasmids
/
Repressor Proteins
/
Transcription, Genetic
/
Tumor Virus Infections
/
Virology
/
Urinary Bladder
/
Urinary Bladder Neoplasms
/
Cell Line, Transformed
/
Transfection
Type of study:
Prognostic_studies
Limits:
Humans
Language:
Zh
Journal:
Acta Academiae Medicinae Sinicae
Year:
2004
Type:
Article