Enhanced action of a BTB/POZ domain protein on the expression of hsp90alpha gene in heat shock / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 392-395, 2004.
Article
in Zh
| WPRIM
| ID: wpr-231922
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of a BTB/POZ domain protein in the expression of hsp90alpha gene.</p><p><b>METHODS</b>The eukaryotic expression plasmids of sense- and antisense-GAGA related protein (GRP) or empty vector were transfected into Jurkat cells with pREP4 episomal vector plasmids carrying the hsp90alpha promoter sequence from -1756 to +37 and control plasmids pMCAT. Total RNA was extracted. The relative promoter activity of hsp90alpha-CAT reporter gene was determined by competitive RT-PCR assay.</p><p><b>RESULTS</b>GRP markly increased the relative promoter activity of hsp90alpha-CAT reporter gene during heat shock.</p><p><b>CONCLUSION</b>GRP may promote the expression of hsp90alpha gene by participating in chromatin remolding.</p>
Full text:
1
Index:
WPRIM
Main subject:
Repressor Proteins
/
Transcription Factors
/
DNA
/
Chemistry
/
Gene Expression Regulation
/
Cloning, Molecular
/
DNA, Complementary
/
Homeodomain Proteins
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HSP90 Heat-Shock Proteins
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Heat-Shock Response
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Acta Academiae Medicinae Sinicae
Year:
2004
Type:
Article