Establishment of a drug screening model for identifying up-regulator of human high density lipoprotein receptor / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 354-358, 2004.
Article
in Zh
| WPRIM
| ID: wpr-231929
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a new drug screening model based on transcriptional regulation of human high density lipoprotein (HDL) receptor gene CD36 and LIMPII analogous-1 (CLA-1) for discovering up-regulator of this receptor.</p><p><b>METHODS</b>The upstream regulatory sequence of CLA-1 was obtained by polymerase chain reaction. A recombinant reporter plasmid pGL3-CLAP was constructed by inserting the regulatory sequence upstream of luciferase gene of pGL3-Basic. Human hepatoma cell line BEL-7402 was transfected with pGL3-CLAP. Samples were detected by testing luciferase activity of transfected BEL-7402 cells in microtiter wells.</p><p><b>RESULTS</b>The drug screening model was established and optimized. Significant difference was present between pGL3-CLAP and pGL3-Basic transfected BEL-7402 cells (P< 0.001), and coefficient of variation was less than 10%. After primary and secondary screening, 1 compounds and 3 fermentation extracts had up-regulating activities.</p><p><b>CONCLUSION</b>This new drug screening model may be efficiently used to screen up-regulators of human HDL receptor expression, which might become lead compounds for new anti-atherosclerosis drugs.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Transcription, Genetic
/
RNA, Messenger
/
Receptors, Immunologic
/
Up-Regulation
/
Gene Expression Regulation
/
Cholesterol Esters
/
RNA-Binding Proteins
/
Receptors, Lipoprotein
/
CD36 Antigens
Type of study:
Diagnostic_studies
/
Screening_studies
Limits:
Humans
Language:
Zh
Journal:
Acta Academiae Medicinae Sinicae
Year:
2004
Type:
Article