Study on the carbapenemase genotype and molecular epidemiology of Acinetobacter baumannii / 中华流行病学杂志
Chinese Journal of Epidemiology
; (12): 381-384, 2007.
Article
in Zh
| WPRIM
| ID: wpr-232297
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate antibiotic resistance, clonal relatedness and carbapenemase genotype among carbapenem-resistant Acinetobacter baumannii collected from 3 comprehensive hospitals in Ningbo city, Zhejiang province.</p><p><b>METHODS</b>28 strains of carbapenem resistant Acinetobacter baumannii were collected from Ningbo Li Hui-li Hospital, Ningbo Li Hui-li Hospital, Ningbo First Hospital, and N ingbo Second Hospital. The minimum inhibitory concentrations (MIC) of these strains were examined by agar dilution and E-test method. Homology of these isolates was analyzed by pulse-field gel electrophoresis (PFGE) and Genotype of carbapenemases were analyzed by PCR and verified by DNA sequencing.</p><p><b>RESULTS</b>28 strains of Acinetobacter baumanii were highly resistant to all of the antibiotics except polymyxin E. They were classified into 4 clones based on PFGE pattern. Clone A and B had been spreading widely. All of the 28 strains produced carbapenemases which were confirmed as OXA-23 by PCR and sequencing. Metallo-beta-lactamase was not detected in any of the isolates.</p><p><b>CONCLUSION</b>All of t hecarbapenem-resistant Acinetobacter baumannii collected from Ningbo were producing OXA-23 carbapenemase, suggesting that the transmission of clones had occurred in the 3 hospitals.</p>
Full text:
1
Index:
WPRIM
Main subject:
Bacterial Proteins
/
Beta-Lactamases
/
Acinetobacter Infections
/
Drug Resistance, Microbial
/
China
/
Polymerase Chain Reaction
/
Epidemiology
/
Molecular Epidemiology
/
Acinetobacter baumannii
/
Drug Therapy
Type of study:
Screening_studies
Limits:
Humans
Country/Region as subject:
Asia
Language:
Zh
Journal:
Chinese Journal of Epidemiology
Year:
2007
Type:
Article