Curcumin inhibits HeLa cell invasion and migration by decreasing inducible nitric oxide synthase / 南方医科大学学报
Journal of Southern Medical University
; (12): 1752-1756, 2013.
Article
in Zh
| WPRIM
| ID: wpr-232709
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibitory effects of curcumin against HeLa cell invasion and migration and explore the underlying mechanisms.</p><p><b>METHODS</b>HeLa cells were exposed to curcumin treatment at the concentrations of 0, 10, 25, 50, 100, 150 and 200 µmol/L for 24 h. MTT and TUNEL assays were used to assess the cell proliferation inhibition and apoptosis, respectively. Transwell assay was used to evaluate the invasiveness and migration of the treated cells, and RT-PCR and Western blotting were employed to detect the changes in the expression of inducible nitric oxide synthase (iNOS), and MMP-9 and E-cad, the 2 markers of cell invasion and migration, were detected by Western blotting. The capacity of NO production in HeLa cells was measured by Griess method.</p><p><b>RESULTS</b>Curcumin inhibited the proliferation of HeLa cells by inducing cell apoptosis in a concentration-dependent manner. Curcumin inhibited the invasion and migration of HeLa cells by increasing E-cad expression and decreasing MMP-9 expression, and also decreased the expression level of iNOS and NO production in the cells.</p><p><b>CONCLUSION</b>Curcumin inhibits the invasion and migration of HeLa cells by decreasing the expression of iNOS.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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HeLa Cells
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Cell Movement
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Apoptosis
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Matrix Metalloproteinase 9
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Curcumin
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Cell Proliferation
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Nitric Oxide Synthase Type II
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Metabolism
Limits:
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2013
Type:
Article