Sequence analysis of housekeeping genes recA, dnaE, and mdh in different serogroups or biotypes of Vibrio cholerae isolated from China / 南方医科大学学报
Journal of Southern Medical University
; (12): 1720-1723, 2006.
Article
in Zh
| WPRIM
| ID: wpr-232798
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyze sequences of the housekeeping genes including recA, dnaE, and mdh in different serogroups or different biotypes of Vibrio cholerae (VC) strains isolated from China.</p><p><b>METHODS AND RESULTS</b>recA, dnaE, and mdh genes of Vibrio cholerae were obtained by PCR, sequenced and analyzed. Forty-four variable bases were identified in the 500 bases of recA gene of 18 VC strains, and the mutation of only 3 variable bases could result in changes of 2 amino acids. In the 600 bases of dnaE genes of 18 strains, 32 variable bases were found and only 1 contributed to an amino acid change. In the 367 bases of mdh genes of 18 strains, only 1 variable base was identified whose mutation involved an amino acid convertion. Toxic EI Tor biotype (EVC) strains and toxic O139 serogroup strains were closely related. Non-toxic strains of different serogroups or types were lowly related. Non-toxic and toxic strains of different serogroups or types were lowly related.</p><p><b>CONCLUSION</b>Toxic EVC and toxic O139 serogroup strains isolated from different areas of China may evolve from a common original strain, and toxic O139 VC strains may come from toxic EVC strains.</p>
Full text:
1
Index:
WPRIM
Main subject:
Phylogeny
/
Rec A Recombinases
/
Bacterial Proteins
/
Vibrio cholerae
/
Molecular Sequence Data
/
Base Sequence
/
Sequence Homology, Nucleic Acid
/
China
/
Classification
/
Sequence Analysis, DNA
Type of study:
Prognostic_studies
Limits:
Humans
Country/Region as subject:
Asia
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2006
Type:
Article