Regulation of hepatic stellate cell activation by interleukin-10/platelet derived growth factor/mitogen-activated protein kinase pathway / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 35-37, 2005.
Article
in Zh
| WPRIM
| ID: wpr-233628
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the regulatory effect of interleukin-10 (IL10) on the activation of hepatic stellate cells (HSC) through platelet derived growth factor (PDGF) and mitogen-activated protein kinase (MAPK) pathways.</p><p><b>METHODS</b>HSC were divided randomly into 4 groups. Group 1 served as a control. HSC were incubated with 1 ng/ml, 5 ng/ml, and 25 ng/ml IL-10 in groups 2, 3 and 4. RT-PCR and western blot were used to detect the expression of PDGF and MAPK protein ERK and p38 and alpha-SMA.</p><p><b>RESULTS</b>Compared with the control group, expressions of ERK, p38 and alpha-SMA of groups 2, 3 and 4 were significantly lower (F values were 240.47, 21.39, 28.86 respectively. IL-10 inhibited PDGF and MAPK protein ERK and p38 and alpha-SMA expression in a dose-dependent way.</p><p><b>CONCLUSION</b>IL-10 inhibits activation of HSC through the PDGF/MAPK pathway.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Platelet-Derived Growth Factor
/
Signal Transduction
/
Cell Line
/
Interleukin-10
/
Mitogen-Activated Protein Kinases
/
Hepatocytes
/
Cell Biology
/
Cell Proliferation
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Hepatology
Year:
2005
Type:
Article