Inhibition of transcription and expression of X gene by lamivudine / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 27-30, 2005.
Article
in Zh
| WPRIM
| ID: wpr-233630
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>The X protein of the hepatitis virus B plays an important role in the development of hepatocellular carcinoma (HCC). In this experiment we studied the effects of lamivudine on replication, transcription and expression of the X gene.</p><p><b>METHODS</b>The replication of transfected X gene was measured by polymerase chain reaction. The mRNA of transfected X gene was analyzed by reverse transcriptase polymerase chain reaction. The expression of X protein was detected by biosensor. Moreover, we studied if these effects changed in a dose and time dependent manner.</p><p><b>RESULTS</b>The OD data of purpose band reflected the replication of X gene for control and lamivudine groups were 151.4+/-3.5 and 144.0+/-11.4, respectively. The transcription of mRNA for X gene could be inhibited by lamivudine at a concentration of 4.36 x 10(-4) mol/L for 24 h. The OD data of purpose band for control and treatment groups (16 h) were 243.9+/-9.0 and 133.2+/-7.8. The inhibition was enhanced with the increase of lamivudine concentration and reacting time. The expression of the X protein in HepG2x cells was suppressed by lamivudine at a concentration of 4.36 x 10(-4) mol/L for 16 hours. The resonance unit for expression of the X protein decreased from 353.3+/-15.9 to 252.3+/-18.8.</p><p><b>CONCLUSION</b>Although the replication of transfected X gene of HepG2x cells is not influenced by lamivudine, it may significantly inhibit the transcription and expression of the X protein.</p>
Full text:
1
Index:
WPRIM
Main subject:
Transcription, Genetic
/
Virology
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Gene Expression Regulation, Neoplastic
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Trans-Activators
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Hepatitis B virus
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Carcinoma, Hepatocellular
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Reverse Transcriptase Inhibitors
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Lamivudine
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Therapeutic Uses
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Drug Therapy
Limits:
Female
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Humans
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Male
Language:
Zh
Journal:
Chinese Journal of Hepatology
Year:
2005
Type:
Article