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siRNA targeted against survivin induces apoptosis of pancreatic cancer cells / 南方医科大学学报
Article in Zh | WPRIM | ID: wpr-234169
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of a sequence-specific small interfering RNA (siRNA) in suppressing survivin expression and cell proliferation and inducing apoptosis of PC-2 cells.</p><p><b>METHODS</b>The plasmid expression vector of siRNA targeted against survivin was constructed and transfected into PC-2 cells with Lipofectamine 2000. The changes of survivin expression were detected by semi-quantitative RT-PCR and immunohistochemical SP methods. The effect of siRNA in suppressing the proliferation of PC-2 cells was detected by MTT assay, and its role in inducing PC-2 cell apoptosis evaluated by flow cytometry.</p><p><b>RESULTS</b>The sequence-specific siRNA effectively suppressed survivin expression at both mRNA and protein levels with inhibition rate of 81.25% at mRNA level and 74.24% at protein level. Survivin expression suppression significantly inhibited the proliferation of PC-2 cells, and at 24 and 48 h after cell seeding, the proliferation inhibition rate was 28.00% and 33.38% respectively; 24, 48 h after the transfection, apoptosis occurred in 8.46% and 7.53% of the cells, respectively.</p><p><b>CONCLUSIONS</b>The plasmid expression vector for the siRNA against survivin constructed in the study can effectively and specifically suppress survivin expression in PC-2 cells, and blocking survivin expression suppresses PC-2 cell proliferation and induces cell apoptosis. siRNA targeted against survivin has a potential value in gene therapy for pancreatic cancer.</p>
Subject(s)
Full text: 1 Index: WPRIM Main subject: Pancreatic Neoplasms / Pathology / RNA, Messenger / Transfection / Cell Survival / Apoptosis / RNA, Small Interfering / RNA Interference / Cell Line, Tumor / Cell Proliferation Limits: Humans Language: Zh Journal: Journal of Southern Medical University Year: 2006 Type: Article
Full text: 1 Index: WPRIM Main subject: Pancreatic Neoplasms / Pathology / RNA, Messenger / Transfection / Cell Survival / Apoptosis / RNA, Small Interfering / RNA Interference / Cell Line, Tumor / Cell Proliferation Limits: Humans Language: Zh Journal: Journal of Southern Medical University Year: 2006 Type: Article