Activation of Rho-kinase pathway is involved in angiotensin II-induced contraction of human airway smooth muscle cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1416-1420, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-235112
ABSTRACT
<p><b>OBJECTIVE</b>To investigate of the regulatory effect of Rho-kinase pathway activation on angiotensin II (Ang II)-induced contraction of human airway smooth muscle cells (HASMCs) in vitro.</p><p><b>METHODS</b>Cultured primary HASMCs were divided into control group, AngII group, AngII + irbesartan group and AngII + Y-27632 group with corresponding treatment. AngII-induced contraction of HASMCs was evaluated using collagen gel lattices and observed morphologically using immunofluorescence assay. Western Blotting was significantly performed to examine the protein expression of Rho-kinase signal pathway.</p><p><b>RESULTS</b>AngII-induced HASMC contraction was inhibited by treatments with irbesartan and Y-27632 as shown by gel contraction assay (P<0.001). Y-27632 treatment produced a stronger inhibitory effect than irbesartan on the expression of phosphorylated moesin, a substrate of Rho kinase (P<0.05).</p><p><b>CONCLUSION</b>AngII induces the contraction of HASMCs partially as a result of activation of Rho-kinase pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Pyridines
/
Asthma
/
Tetrazoles
/
Biphenyl Compounds
/
Bronchi
/
Angiotensin II
/
Signal Transduction
/
Cell Biology
/
Rho-Associated Kinases
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2011
Type:
Article
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