Preparation and quality control of soybean isoflavone dropping pills / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish the optimized preparation procedure and study the method to determine the content for soybean isoflavone(SIF) Dropping Pills.</p><p><b>METHOD</b>The preparation conditions, such as the proportion between SIF and PEGs, the temperature of mixture of SIF and PEGs, dropping distance, etc., were studied with Uniform Design and One-way ANOVA. SIF was identified by TLC and the content of SIF was determined by UV spectrometry at 262 nm detection wavelength.</p><p><b>RESULT</b>Three batches of the prepared products meet the standards of the Chinese pharmacopoeia on dropping pills. SIF can be identified by TLC. Using UV spectrometry, the linear range of SIF was 0. 407 2 to 4. 072 g x mL(-1) and the correlation coefficient was 0. 999 8. In high, middle and low concentration, average recovery were 96. 54%, 97.27% and 97.21%, respectively (RSD were 1.3%, 0.78% and 0.71%).</p><p><b>CONCLUSION</b>The preparation procedure is feasible, simple and suitable, the method established in this paper can be adopted for the quality control of SIF dropping pills, and the determination method is simple, relatively fast and accurate.</p>