Development of an enzyme-linked immunosorbent assay for determination of the furaltadone etabolite, 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in animal tissues / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 449-457, 2012.
Article
in English
| WPRIM
| ID: wpr-235518
ABSTRACT
<p><b>OBJECTIVE</b>To determine 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) residues released from protein bound AMOZ in animal tissues.</p><p><b>METHODS</b>Polyclonal and monoclonal antibodies were produced in this study. A rapid, sensitive, and specific competitive direct enzyme-linked immunosorbent assay (cdELISA) was developed.</p><p><b>RESULTS</b>Rabbit polyclonal antibodies were used in the optimized cdELISA method, and exhibited negligible cross-reactivity with other compounds structurally related to AMOZ. The IC(50) of the polyclonal antibody was 0.16 ng/mL. The method limit of detection in four different types of animal and fish tissues was less than 0.06 μg/kg. Recoveries ranged from 80% to 120% for fortified samples with the coefficient of variation values less than 15%. The results of the cdELISA method were in good agreement with the results from an established liquid chromatography-tandem mass spectrometry confirmatory method used for AMOZ residues.</p><p><b>CONCLUSION</b>The cdELISA method developed in the present study is a convenient practical tool for screening large numbers of animal and fish tissue samples for the the detection of released protein bound AMOZ residues.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Enzyme-Linked Immunosorbent Assay
/
Molecular Structure
/
Chemistry
/
Morpholines
/
Oxazolidinones
/
Methods
/
Nitrofurans
Limits:
Animals
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2012
Type:
Article
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