The role of arecoline on hepatic insulin resistance in type 2 diabetes rats / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 208-212, 2014.
Article
in Zh
| WPRIM
| ID: wpr-236345
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of arecoline on hepatic insulin resistance in type 2 diabetes rats and to elucidate its possible mechanism.</p><p><b>METHODS</b>Forty five Wistar rats were fed with high fructose diet for 12 weeks to induce type 2 diabetic rat model. rats were randomly divided into 5 groups (n = 8) control group, model group and model group were treated with different dose (0, 0.5, 1, 5 mg/kg) of arecoline. After 4 weeks, the fasting blood glucose, blood lipid and insulin level measured , mRNA expression of liver constitutive androstane receptor (CAR), pregnane X receptor (PXR), glucose-6-phosphatase (G6Pase), phosphoenolpyruvate carboxykinase (PEPCK), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) were detected by reverse transcription polymerase chain reaction (RT-PCR), the protein expression of p-AKT and glucose transporter4 (GLUT4) were detected by Western blot.</p><p><b>RESULTS</b>1.5 mg/kg arecoline could significantly decrease the level of fasting blood glucose, blood lipid, blood insulin level and liver G6Pase, PEPCK, IL-6, TNF-alpha mRNA level in type 2 diabetes rats. 1.5 mg/kg arecoline also could significantly increase CAR, PXR mRNA level and p-AKT and GLUT4 protein expression.</p><p><b>CONCLUSION</b>Arecoline improved hepatic insulin resistance in type 2 diabetes rats by increasing the mRNA levels of CAR and PXR leading to the creased glucose metabolism and inflammation related genes expression.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Phosphoenolpyruvate Carboxykinase (GTP)
/
Arecoline
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Insulin Resistance
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Receptors, Steroid
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Interleukin-6
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Tumor Necrosis Factor-alpha
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Rats, Wistar
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Receptors, Cytoplasmic and Nuclear
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Glucose-6-Phosphatase
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Applied Physiology
Year:
2014
Type:
Article