Application of multiplex rt-PCR assay for screening rare or cryptic chromosome translocations in de novo patients with acute myeloid leukemia / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1138-1142, 2010.
Article
in Zh
| WPRIM
| ID: wpr-237578
Responsible library:
WPRO
ABSTRACT
This study was aimed to investigate the clinical feasibility of using multiplex PT-PCR assay for screening rare/cryptic chromosome translocations in patients with de novo acute myeloid leukemia. For 126 patients with de novo AML-M4/M5 without common chromosome translocations including t(15;17), t(8;21) and t(16;16), 3 parallel multiplex RT-PCR assays were set up to detect 6 mll-related gene rearrangements (mll/af10, mll/af17, mll/ell, mll/af9, mll/af6 and mll/enl) with low detection rate and 4 rare fusion genes (dek/can, tls/erg, aml1/mds (evi1) and npm/mlf1). The results showed that 11 patients with positive result from 126 patients were detected which involved in 5 molecular abnormalities. Among them, 10 cases were AML-M5 (16.67%), 1 cases AML-M4 (1.51%). The marker chromosomes were observed in 2 cases out of 11 cases through conventional karyotyping analysis, the karyotyping analysis in 1 case was not performed because this case had 1 mitotic figure only, no any cytogenetic aberrations were found in other 8 cases through R-band karyotyping analysis. It is concluded that multiplex RT-PCR designed in this study can quickly, effectively and accurately identify the rare/cryptic chromosome translocations and can be used in clinical detection.
Full text:
1
Index:
WPRIM
Main subject:
Translocation, Genetic
/
Gene Rearrangement
/
Leukemia, Myeloid, Acute
/
Genetic Testing
/
Chromosome Banding
/
Reverse Transcriptase Polymerase Chain Reaction
/
Genetics
/
Methods
Type of study:
Diagnostic_studies
/
Screening_studies
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2010
Type:
Article