Morphological study of thick basement membrane-like layer in chronic rhinosinusitis / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
; (12): 31-34, 2006.
Article
in Zh
| WPRIM
| ID: wpr-239073
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the thick basement membrane-like layer under the damaged epithelium in patients with chronic rhinosinusitis (CRS) and to study its principal components.</p><p><b>METHODS</b>Patients (n = 40) with CRS undergoing functional endoscopic sinus surgery were recruited for the study. Patients (n = 10) with no evidence of sinus disease were used as control subjects. Ethmoidal sinus mucosa specimen were stained by PAS staining to measure the thickness of basement membrane-like layer. Immunohistochemical staining, picrosirius-polarization method and transmission electron microscopy (TEM) were used to analyse the nature of the layer.</p><p><b>RESULTS</b>The thickness of basement membrane-like layer in CRS type I and CRS type II were significantly increased compared with normal control subjects (t = 5. 426, P < 0. 01; t = 8. 375, P < 0.01). The thickness of CRS type II was significantly greater compared with CRS type I (t = 3. 908, P < 0.01). There was no difference between atopic group and inotropic group (t = 0.803, P> 0.05). There was no expression of fibronectin, laminin, IgA, IgG and IgM in the thick basement membrane-like layer. A thick reticular structure containing collagen fibrils and devoid of cellular elements was observed by TEM.</p><p><b>CONCLUSIONS</b>This study demonstrates that a long-standing chronic inflammation leads to deposition of collagens type I , III and IV under the damaged epithelium, resulting in an increasingly thick sub-basement membrane layer.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Sinusitis
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Basement Membrane
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Case-Control Studies
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Chronic Disease
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Collagen
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Metabolism
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Nasal Mucosa
Type of study:
Observational_studies
Limits:
Adolescent
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Adult
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Aged
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Female
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Humans
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Male
Language:
Zh
Journal:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Year:
2006
Type:
Article