Experimental study on the effect of sensitized sera on engraftment of hematopoietic stem cells / 中华血液学杂志
Chinese Journal of Hematology
; (12): 531-534, 2008.
Article
in Zh
| WPRIM
| ID: wpr-239986
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of sensitized sera on engraftment of hematopoietic stem cells (HSCs) and its mechanism.</p><p><b>METHODS</b>Bone marrow cells (BMCs) from C57BL/6 mice were incubated with sensitized sera (group I) or normal sera (group II), and then washed and transplanted into irradiated BALB/c mice. The survival analysis and engraftment evaluation of the recipients were observed. The incubated BMCs were bound with goat anti mouse IgG for and labeled with Annexin V for apoptosis detection.</p><p><b>RESULTS</b>Seven out of ten recipient mice in group I died of bone marrow failure at day 10 after transplantation, while all of those in group II were long-term survived. Engraftment assay showed recipients blood count and BMCs progressively decreased along with time passing in group I; in addition, the chimeric percentage of donor cells progressively decreased as well. The percentage of BMCs binding with goat anti mouse IgG in group I or group II were (90.3 +/- 5.1)% and (5.2 +/- 2.4)%, respectively, and the difference was statistically significant (P < 0.01). However, no significant difference was found in the apoptosis detection between the two groups (P > 0.05).</p><p><b>CONCLUSION</b>The engraftment capacity of HSCs is significantly impaired by sensitized sera, the antibodies in sensitized sera may bind to antigens expressed on HSCs but do not induce apoptosis.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
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Bone Marrow Cells
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Cells, Cultured
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Apoptosis
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Annexin A5
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Hematopoietic Stem Cell Transplantation
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Immune Sera
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Metabolism
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Mice, Inbred BALB C
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Mice, Inbred C57BL
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Hematology
Year:
2008
Type:
Article