Identification of interaction partners and function analysis of new splicing product of human LMO2 gene / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 325-328, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-240016
ABSTRACT
<p><b>OBJECTIVE</b>To identify the interaction partners of a new splicing product of LMO2 gene (LMO2-C), and study its function in K562 cells.</p><p><b>METHODS</b>Maltose binding protein (MBP) pull down and mammalian two-hybrid assay (MTHA) were used to identify the interaction partners of LMO2-C in K562 cells. Semiquantitative RT-PCR was used to detect the expression of hematopoietic specific gene glycoprotein (GPA) in K562 cells.</p><p><b>RESULTS</b>MBP-LMO2-C fusion protein was expressed and purified in soluble form successfully. Endogenous GATA1 and LDB1 proteins were confirmed to bind to LMO2-C by MBP pull down analysis. The MTHA also showed that LMO2-C had comparable binding affinities to LDB1 with LMO2-L, and over expression of LMO2-C prevented LMO2-L from binding to LDB1, the inhibition rate being (81.13 +/- 0.68)%. RT-PCR results showed that the expression level of GPA was reduced [(51.00 +/- 1.58)%] in K562 cells while LMO2-C overexpressed.</p><p><b>CONCLUSION</b>LMO2-C can bind endogenous GATA1 and LDB1 protein in K562 cells and down regulates the expression of GPA.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transcription Factors
/
RNA Splicing
/
Proto-Oncogene Proteins
/
K562 Cells
/
Two-Hybrid System Techniques
/
Periplasmic Binding Proteins
/
Adaptor Proteins, Signal Transducing
/
DNA-Binding Proteins
/
GATA1 Transcription Factor
/
Maltose-Binding Proteins
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2008
Type:
Article
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