Identification of TGF-beta/Smads pathway in human prostate cancer cell lines and its significance / 中华男科学杂志
National Journal of Andrology
; (12): 920-924, 2009.
Article
in Zh
| WPRIM
| ID: wpr-241230
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine the TGF-beta/Smads signal pathway in different human prostate cancer cell lines, and to explore the role of the TGF-beta/Smads pathway in the progression and metastasis of prostate cancer and its possible mechanisms.</p><p><b>METHODS</b>We detected the expressions of the key proteins involved in the TGF-beta/Smads pathway, TbetaR II, Smad2/3, p-Smad2 and Smad4, in prostate cancer cell lines LNCaP, PC-3, DU145, IF11 and IA8 with different metastatic potentials by Western blotting.</p><p><b>RESULTS</b>TbetaR II was expressed highly in PC-3, DU145, IF11 and IA8, but extremely lowly in LNCaP. Smad2/3 was highly expressed in all the cell lines with different intensity, while p-Smad2 only in PC-3 and DU145. The expression of Smad4 was detected in LNCaP, PC-3 and DU145, but not in IF11 and IA8.</p><p><b>CONCLUSION</b>The status of the TGF-beta/Smads pathway differs in the cell lines with different metastatic potentials, only active in PC-3 and DU145. The TGF-beta/Smads pathway may be involved in the invasion and metastasis of prostate cancer through altering the expressions of the key proteins in it.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Prostatic Neoplasms
/
Signal Transduction
/
Transforming Growth Factor beta
/
Cell Line, Tumor
/
Smad2 Protein
/
Metabolism
Type of study:
Prognostic_studies
Limits:
Humans
/
Male
Language:
Zh
Journal:
National Journal of Andrology
Year:
2009
Type:
Article