Immunosuppressive effects of fetal bone marrow derived mesenchymal stem cells on in vitro proliferation of adult peripheral lymphocyte and expression of immune-related factors / 中华血液学杂志
Chinese Journal of Hematology
; (12): 891-896, 2014.
Article
in Zh
| WPRIM
| ID: wpr-242032
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the potential immunomodulatory properties of fetal bone marrow derived mesenchymal stem cells (FBM- MSCs).</p><p><b>METHODS</b>Mononuclear cells from the bone marrow of second trimester (14-22 wks) fetus were isolated and cultured for the derivation of MSCs. The derived FBM-MSC cells were characterized via morphology, immunophenotyping and the adipogenic and osteogenic differentiation assays. The immunomodulatory properties of FBM-MSC on lymphocytes were evaluated through the co- culture assay with PHA activated adult peripheral blood mononuclear cells (PBMCs).</p><p><b>RESULTS</b>Derived FBM-MSCs were CD29⁺, CD44⁺, CD49e⁺, CD73⁺, CD90⁺, CD105⁺ and CD31⁻ , CD34⁻ , CD45⁻ , HLA-DR⁻ and can be differentiated into adipocytes and osteocytes. When co-cultured with PHA-activated PBMCs, FBM-MSCs inhibited the proliferation of lymphocytes up to 96% and down-regulated the secretion of inflammatory cytokines such as IFN-γ and TNF-α up to 90.9% and 58.4% respectively. When compared with FBM-MSCs cultured alone, the expression of MSCs derived immunomodulatory cytokines, such as IDO, TSG-6 and TGF-β, was up-regulated significantly in the co-culture system.</p><p><b>CONCLUSION</b>MSC derived from fetal bone marrow demonstrated immunosuppressive effects on adult PBMCs in vitro. MSC-derived cytokines like IDO, TSG-6 and TGF-β may be critical for FBM-MSCs mediated immunosuppressive function.</p>
Full text:
1
Index:
WPRIM
Main subject:
Osteogenesis
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Bone Marrow
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In Vitro Techniques
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Bone Marrow Cells
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Hematopoietic Stem Cells
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Leukocytes, Mononuclear
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Lymphocytes
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Cell Differentiation
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Cells, Cultured
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Cytokines
Limits:
Adult
/
Humans
Language:
Zh
Journal:
Chinese Journal of Hematology
Year:
2014
Type:
Article