Effect of co-expression of nicotinic acid phosphoribosyl transferase and pyruvate carboxylase on succinic acid production in Escherichia coli BA002 / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1855-1859, 2013.
Article
in Zh
| WPRIM
| ID: wpr-242446
Responsible library:
WPRO
ABSTRACT
Escherichia coli BA002, in which the ldhA and pflB genes are deleted, cannot utilize glucose anaerobically due to the inability to regenerate NAD+. To restore glucose utilization, overexpression of nicotinic acid phosphoribosyltransferase (NAPRTase) encoded by the pncB gene, a rate-limiting enzyme of NAD(H) synthesis pathway, resulted in a significant increase in cell mass and succinate production under anaerobic conditions. However, a high concentration of pyruvate was accumulated. Thus, co-expression of NAPRTase and the heterologous pyruvate carboxylase (PYC) of Lactococcus lactis subsp. cremoris NZ9000 in recombinant E. coli BA016 was investigated. Results in 3 L fermentor showed that OD600 is 4.64 and BA016 consumed 35.00 g/L glucose and produced 25.09 g/L succinate after 112 h under anaerobic conditions. Overexpression of pncB and pyc in BA016, the accumulation of pyruvic acid was further decreased, and the formation of succinic acid was further increased.
Full text:
1
Index:
WPRIM
Main subject:
Pentosyltransferases
/
Pyruvate Carboxylase
/
Industrial Microbiology
/
Genetic Engineering
/
Lactococcus lactis
/
Succinic Acid
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Escherichia coli
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Fermentation
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Genetics
/
Glucose
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2013
Type:
Article