Knockdown of survivin expression by small interfering RNA suppresses proliferation of two human cancer cell lines / 中国医学科学杂志(英文版)
Chin. med. sci. j
; Chin. med. sci. j;(4): 115-119, 2006.
Article
in En
| WPRIM
| ID: wpr-243605
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct an expression vector of small interfering RNA (siRNA) against survivin and observe its effects on survivin expression and proliferation of human pancreatic cancer cell line PC-2 and breast cancer cell line MCF-7.</p><p><b>METHODS</b>Constructed an expression vector of siRNA against survivin and transfected it into PC-2 and MCF-7 cells using lipofectamine 2000. The expression of survivin was detected by semi-quantitive RT-PCR and immunohistochemistry, and its effects on proliferation of PC-2 and MCF-7 cells were detected by MTT assay.</p><p><b>RESULTS</b>The introduction of sequence-specific siRNA could efficiently suppress survivin expression at both mRNA and protein levels in the two cancer cell lines. In PC-2 cells, the expression inhibition rates were 81.25% at mRNA level and 74.24% at protein level. In MCF-7 cells, the expression inhibition rates were 64.91% at mRNA level and 79.72% at protein level. The proliferation of PC-2 and MCF-7 cells was also suppressed, and 24 and 48 hours after the cells were reseeded, the proliferation inhibition rates of PC-2 cells were 28.00% and 33.38%, and that of MCF-7 cells were 31.58% and 33.02%, respectively.</p><p><b>CONCLUSIONS</b>The expression vector of siRNA against survivin can block survivin expression in PC-2 and MCF-7 cells efficiently and specifically. Down regulation of survivin expression can suppress proliferation of PC-2 and MCF-7 cells. Survivin RNAi may have potential value in gene therapy of human cancers.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pancreatic Neoplasms
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Pathology
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Plasmids
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Therapeutics
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Breast Neoplasms
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RNA, Messenger
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RNA, Neoplasm
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Base Sequence
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Transfection
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Gene Expression
Limits:
Female
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Humans
Language:
En
Journal:
Chin. med. sci. j
Year:
2006
Type:
Article