Effect of andrographolide on Candida albicans biofilm dispersion / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3339-3343, 2014.
Article
in Zh
| WPRIM
| ID: wpr-244568
Responsible library:
WPRO
ABSTRACT
Along with the increase in fungal infections, Candida albicans prevention and control become the focus of anti-fungal infection at present. This study aims to discuss the effect monomer andrographolide (AG) on C. albicans biofilm dispersion. In the experiment, micro-well plates and medical catheter pieces were used to establish the C. albicans biofilm model. It was discovered by XTT assay and flat band method that 1 000, 500, 250 mg x L(-1) AG could impact the activity of C. albicans biofilm dispersion cells. The morphological structures of residual biofilms on catheter pieces were observed with scanning electron microscopy, which showed that 1 000, 500, 250 mg x L(-1) AG could induce C. albicans biofilm dispersion in a dose-dependent manner, and the dispersed cells were dominated by the yeast phase. According to the real-time fluorescence quantification PCR (qRT-PCR) test, AG could up-regulate HSP90 expression and down-regulate UME6 and PES1 expressions. This study demonstrates that AG could induce C. albicans biofilm dispersion to some extent.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Physiology
/
Time Factors
/
Candida albicans
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Fungal Proteins
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Microscopy, Electron, Scanning
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Gene Expression Regulation, Fungal
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HSP90 Heat-Shock Proteins
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Biofilms
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Reverse Transcriptase Polymerase Chain Reaction
Type of study:
Prognostic_studies
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2014
Type:
Article