Influence of TLR2 and TLR4 agonists on migration of cord blood CD34(+) cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 469-472, 2011.
Article
in Zh
| WPRIM
| ID: wpr-244900
Responsible library:
WPRO
ABSTRACT
This study was aimed to investigate the influence of TLR2 and TLR4 agonists on the migration and adhesion activity of umbilical cord blood (UCB) CD34(+) cells and to explore the underlying mechanism. The expression of TLR2 and TLR4 on UCB CD34(+) cells was detected with flow cytometry. The effect of TLR2 agonist (PAM3CSK4) and TLR2 agonist (LPS) on the migration and adhesion ability of UCB CD34(+) cells was evaluated with chemotaxis and adhesion assays. The results indicated that expression levels of TLR2 and TLR4 were (14.2 ± 3.8)%, (19.6 ± 4.1)% respectively. Compared with the control group, the migration activity of UCB CD34(+) cells toward SDF-1 decreased significantly in LPS group (p < 0.01). The adhesion activity was not altered significantly in LPS group. However, both the migration activity towards SDF-1 and the adhesion activity of UCB CD34(+) cells were not changed significantly in PAM3CSK4 group. Further study found that LPS did not affect the expression level of CXCR4 on CD34(+) cells, but could inhibit the spontaneous migration ability of CD34(+) cells. It is concluded that TLR4 activation can decrease the chemotaxis function of CD34(+) cells towards SDF-1, which may associate with the decreased spontaneous migration ability of CD34(+) cells.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Blood
/
Cell Movement
/
Cells, Cultured
/
Lipopolysaccharides
/
Antigens, CD34
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Cell Biology
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Allergy and Immunology
/
Toll-Like Receptor 2
/
Toll-Like Receptor 4
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2011
Type:
Article