Isolation of mesenchymal stem cells from bone marrow filters by primary explant culture / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 459-463, 2011.
Article
in Zh
| WPRIM
| ID: wpr-244902
Responsible library:
WPRO
ABSTRACT
This study was aimed to investigate whether mesenchymal stem cells (MSC) can be isolated from bone marrow filters which have always been discarded. The bone marrow (BM) particles from BM filters of 2 healthy donors were cultivated by primary explant culture. After expansion, the number of MSC was counted and their immunophenotype and differentiation potential were detected. The results indicated that many MSC were found in bone marrow particles from filters, and nearly 10(7) MSC were obtained at 3 passages of expansion. They not only possessed the characteristics of morphology and immunophenotype of MSC, but also could differentiate into osteoblasts, chondrocytes and adipocytes. In conclusion, a large amount of MSC can be obtained from BM filters if the BM particles were cultivated by primary explant culture.
Full text:
1
Index:
WPRIM
Main subject:
Bone Marrow Cells
/
Cell Differentiation
/
Cell Separation
/
Cells, Cultured
/
Cell Biology
/
Tissue Culture Techniques
/
Mesenchymal Stem Cells
/
Methods
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2011
Type:
Article