Effect and mechanism of total flavonoids of bugloss on rats with myocardial ischemia and reperfusion injury / 药学学报
Acta Pharmaceutica Sinica
; (12): 875-881, 2014.
Article
in Zh
| WPRIM
| ID: wpr-245000
Responsible library:
WPRO
ABSTRACT
This study is to investigate the effect of total flavonoids of Uygur medicine bugloss (BTF) on rats with myocardial ischemia/reperfusion injury, and to explore the mechanisms by which it acts. Left anterior descending (LAD) coronary artery in rats was occluded for 30 min followed by 4 h reperfusion. Meanwhile, BTF dissolved in saline was administered intraperitoneally at dosage of 10, 30 and 50 mg x kg(-1). Electrocardiograph, infarction index, serum myocardial enzymes and heart function were determined to evaluate the effect of BTF. Some other observations were carried out to explore whether inhibiting inflammation and apoptosis is involved in the mechanisms underlying BTF. Our results showed that in ischemia/reperfusion injured rats BTF could dose-dependently reduce myocardial infarction index and myocardial enzyme leakage, and enhance heart function, indicating that it possesses significant cardio protection. ELISA analysis showed that BTF could decrease the content of myocardial inflammatory cytokines such as IL-1beta, IL-6 and TNF-alpha. Western-blotting confirmed that BTF could increase the expression of anti-apoptotic protein Bcl-2 and reduce the expression of proapoptosis protein Bax. Further more, the phosphorylation level of PI3K and Akt was upregulated by BTF treatment. BTF can protect rat against myocardial ischemia/reperfusion injury. Anti-inflammation and inhibition of apoptosis through upregulating PI3K/Akt signal pathway may contribute to the protective effect of BTF.
Full text:
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Index:
WPRIM
Main subject:
Pharmacology
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Phosphorylation
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Flavonoids
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Myocardial Reperfusion Injury
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Signal Transduction
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Chemistry
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Interleukin-6
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Tumor Necrosis Factor-alpha
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Apoptosis
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Phosphatidylinositol 3-Kinases
Limits:
Animals
Language:
Zh
Journal:
Acta Pharmaceutica Sinica
Year:
2014
Type:
Article