Role of silencing PRL-3 expression by miRNA interference in the growth of gastric cancer / 中华外科杂志
Chinese Journal of Surgery
; (12): 618-621, 2008.
Article
in Zh
| WPRIM
| ID: wpr-245543
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of silencing PRL-3 expression by miRNA interference in gastric cancer growth.</p><p><b>METHODS</b>RNA interference mediated by recombinant lentivirus expressing artificial PRL-3 miRNA was employed to knockdown PRL-3 expression in human SGC7901 gastric cancer cells. MTT assay and tumor implantation experiment were conducted to determine the role of PRL-3 in the proliferation of SGC7901 cells and the tumor growth.</p><p><b>RESULTS</b>Transfection of recombinant lentivirus expressing artificial PRL-3 miRNA significantly suppressed the proliferation of SGC7901 cells in vitro. The implanted tumor size of the PRL-3 transfection group was (1.92 +/- 0.18) cm3, significantly smaller than those in control groups [(4.74 +/- 0.39) cm3] (P < 0.05).</p><p><b>CONCLUSIONS</b>Silencing of PRL-3 significantly suppressed the proliferation of SGC7901 cells and tumor growth in vivo. PRL-3 could be a potential therapeutic target in gastric cancer.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Stomach Neoplasms
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Therapeutics
/
Transfection
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Protein Tyrosine Phosphatases
/
Lentivirus
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Xenograft Model Antitumor Assays
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MicroRNAs
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RNA Interference
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Cell Line, Tumor
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Chinese Journal of Surgery
Year:
2008
Type:
Article